Ramirez wasn't in the starting lineup on Sunday night after phoning manager Terry Francona to tell him of a sore right hamstring.
When Ramirez reached for first base on a play in Saturday's game, Francona said it looked awkward.
"He called me this morning and said, 'You know what, it's kind of sore,'" Francona said. "I don't know if Manny would have done that four years ago. That helps me to right away get hold of [Jacoby] Ellsbury and tell him he's playing.
"[Ramirez] is in there getting treatment. He'll be OK, but he's a little sore."
Ramirez was available to pinch-hit, so when the Red Sox rallied to score two and get the tying run on base against Twins closer Joe Nathan in the ninth, Francona sent him to the plate. After Coco Crisp stole second, Ramirez grounded out to shortstop to end the game.
Ramirez hit his 497th career homer on Tuesday in Detroit. He is 9-for-51 at the plate in his last 13 games to go from .370 to .301.
Robert Falkoff is a reporter for MLB.com. This story was not subject to the approval of Major League Baseball or its clubs.