Cody Ross was bumped up to the cleanup hole, with David Ortiz hitting fifth.
This is the first time Youkilis has batted lower than fifth in Boston's batting order since July 22, 2008.
"Maybe just a little breather [for Youkilis]," said Valentine. "Maybe just a little something different for him. Maybe he gets that one pitch. We've all been there. A bloop away."
Valentine noted how hard Youkilis has been working in the batting cage with hitting coach Dave Magadan.
"He's working hard as anybody has ever worked trying to find it," Valentine said.
Youkilis, who struck out in all four at-bats Tuesday night, blasted a two-run homer off the light stanchion atop the Green Monster in left-center field in his first at-bat in Wednesday's game.