Matsuzaka gave up a hit while walking one and striking out two. He threw 56 pitches, 38 of which were for strikes.
The Red Sox had to stop Matsuzaka's first rehab assignment following his start on May 17 because of an injury to his right trap muscle.
Per terms of the 30-day rehab clock, Matsuzaka must be activated by the Red Sox by June 25, provided he doesn't have another setback.
Matsuzaka last pitched for the Red Sox on May 16, 2011. He underwent Tommy John ligament transfer surgery last June.