Pedroia felt soreness in his ailing right thumb following Tuesday's loss, albeit in a different spot than the torn adductor muscle that has bothered him since May.
"I was fine during the game," Pedroia said Wednesday. "And then after the game I was sore, and then today I'm pretty sore. It's not in the area I first got hurt. I think when I dove for that ball in right field, I don't know what happened. [Manager] Bobby [Valentine] wanted me to not play today, because we have an off-day tomorrow. Hopefully everything [will be OK]."
When the Red Sox return to Boston for Thursday's off-day, Pedroia said he will undergo tests from the team's medical staff.
Which part of Pedroia's thumb is irritated now?
"Like the back side. I don't know. I haven't seen the doctors or anybody," Pedroia said. "I don't know, man. I'm just pretty frustrated. It stinks."
With Pedroia out of the lineup, Ryan Kalish moved to the No. 2 spot in the order. Nick Punto got the nod at second base, with Mauro Gomez playing third base in his first Major League start.
Pedroia missed a week with thumb problems in late May and early June. Since his return, he is hitting .210 with one homer, 12 RBIs and a .282 on-base percentage.
He hopes he will be fine for the weekend series against the Yankees after a couple of days off, and clearance from the doctors.
"I don't know," Pedroia said. "I kind of have a high pain tolerance so I don't know. I hope I'm all right."